Evaluation of Polymorphisms in IL8 and IL16 Genes in Patients with Acute Coronary Syndrome
Lílian Caroliny Amorim Silva
Romário Martins Araújo
Fábia Carla Silva Soares
Roberto Pereira Werkhauser
Sergio Tavares Montenegro
Viviane do Carmo Vasconcelos Carvalho
Silvia Maria Lucena Montenegro
Background: Acute coronary syndrome (ACS) is a cardiovascular disease caused by obstruction of coronary arteries by atheromatous plaque. Susceptibility to this disease may be related to genetic variations, such as single nucleotide polymorphisms (SNPs).
Objective: In this study, we evaluated the relationship between SNPs in IL8 (rs4073; -251 A/T) and IL16 (rs11556218; T/G) genes and SCA in a Brazilian population.
Materials and Methods: A sample of 200 patients with ACS and 50 non-ACS patients hospitalized at the Real Hospital Português, Recife – PE, Brazil, and 220 blood donors (donors) was used. Genotyping was carried out by polymerase chain reaction, and DNA sequencing. Statistical analyzes were performed using the Williams G, Chi-square and Kruskal Wallis tests, using the BioEstat 5.0 program, and the data with a value of p < 0.05 were considered significant.
Results: In the IL8 gene, the AT genotype was the most frequent (p > 0.05) in all three groups. In the IL16 gene, genotypic distributions were different between patients with ACS and the donor group (p = 0.002), with the most frequent G allele in the second group (p = 0.0052). The IL-16 cytokine was higher in donors than in patients with ACS (p = 0.04) and the G (TG + GG) allele had higher values of this cytokine (p = 0.01).
Conclusions: The results demonstrate the important role of the rs11556218 SNP in IL16 gene in SCA, evidencing that the G allele may be associated with a decreased risk of the disease. (Int J Cardiovasc Sci. 2020; 33(3):254-262)
Keywords: Cardiovascular Diseases; Acute Coronary Syndrome; Genotype; Dyslipidemia; Diabetes Mellitus; Obesity; Sedentarism.