IJCS | Volume 33, Nº3, May / June 2020

261 1. Silva HS, Moresco RN. Biomarcadores cardíacos na avaliação da síndrome Coronariana Aguda. Scientia Medica. 2011;21(3):132-42 2. Overbaugh KJ.Acute Coronary Syndrome.Am J Nurs. 2009;109(5):42-52. 3. Cavusoglu E, Marmur JD, Yanamadala S, Chopra V, Hedge S, Nazli A, et al. Elevated baseline plasma IL-8 levels are an independent predictor of long-term all-cause mortality in patients with acute coronary syndrome. Atherosclerosis. 2015;242(2):589-94. 4. Apostolakis S, Vogiatzi K, Amanatidou V, Spandidos DA. Interleukin 8 and cardiovascular disease. Cardiovasc Res. 2009;84(3): 353–60. 5. Vogiatzi K, Apotolaskis S, Voudris V, Thomopoilou S, Kochhiadakis, GE, Spandidos DA. Interleukin 8 and Susceptibility to Coronary Artery Disease: a Population Genetics Perspective. J Clin Immunol. 2008;28(4):329-35. 6. Zhang X, Zhang B, ZhangM, Han Y, Zhao Y, Meng Z, et al. Interleukin-8 gene polymorphism is associated with acute coronary syndrome in the Chinese Han population. Cytokine. 2011;56(2):188-91. 7. Zhang M, Fang T, Wang K, Mei H, Lv Z, Wang F, et al. Association of polymorphisms in interleukin-8 gene with cancer risk: a meta-analysis of 22 case–control studies. Onco Targets Ther. 2016 Jun;9:3727-37. 8. Chen J, Ying XM, Huang XM, Huang P, Yan SC. Association between polymorphisms in selected inflammatory response genes and the risk of prostate cancer. Onco Targets Ther. 2016 Jan;9:223-9. 9. Georgitsi MD, Vitoros V, Panou C, Tsangaris I, Aimoniotou E, Gatselis NK, et al. Individualized significance of the −251 A/T single nucleotide polymorphism of interleukin-8 in severe infections. Eur J ClinMicrobiol Infect Dis. 2016;35(4):563-70. References Silva et al. Polymorphisms in acute coronary syndrome Int J Cardiovasc Sci. 2020; 33(3):254-262 Original Article Study limitations The main limitation of the study refers to the composition of the HEMOPE donor group, since it was not possible to match the ages between this group and the patients. Through literature search, it was possible to observe that the use of blood donors to compose a group of comparisons in genetic studies is adequate, since this group of individuals represents a healthy population in general. Although it is known that the age for donation ranges from 16 to 69 years, and therefore the age of patients with ACS would be within this age group, during the collection of samples it was observed that the age obtained from the group of was less than 62 years. This fact generated a selection bias. Therefore, the HEMOPE donor group was used only for comparison of the genotypic and allelic frequencies using the Williams G test and not to establish the odds ratios for the development of ACS. Acknowledgements The authors thank the core-facilities laboratories of Institute Aggeu Magalhães, Fundação Oswaldo Cruz (Fiocruz - PE) for the use of its technology. Author contributions Conception and design of the research: Silva LCA, Werkhauser RP, Carvalho VCV, Montenegro SML. Acquisition of data: Silva LCA, Araújo RM, Soares FCS, Montenegro ST, Carvalho VCV, Montenegro SML. Analysis and interpretation of the data: Silva LCA, Araújo RM, Werkhauser RP, Tashiro T, Carvalho VCV, Montenegro SML. Statistical analysis: Silva LCA, Werkhauser RP, Tashiro T, Carvalho VCV, Montenegro SML. Obtaining financing: Silva LCA, Carvalho VCV, Montenegro SML. Writing of the manuscript: Silva LCA, Carvalho VCV, Montenegro SML. Critical revision of the manuscript for intellectual content: Silva LCA, Araújo RM, Soares FCS, Werkhauser RP, Montenegro ST, Tashiro T, Carvalho VCV, Montenegro SML. Potential Conflict of Interest No potential conflict of interest relevant to this article was reported. Sources of Funding This study was funded by Proep APQ 1620 4.01/15. Study Association This article is part of the thesis of master submitted by Lílian Amorim, from Instituto Aggeu Magalhães (IAM/ FIOCRUZ). Ethics approval and consent to participate This study was approved by the Ethics Committee of the PROCARDIO/REALCOR under the protocol number 565.461. All the procedures in this study were in accordance with the 1975 Helsinki Declaration, updated in 2013. Informed consent was obtained from all participants included in the study.

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