IJCS | Volume 33, Nº3, May / June 2020

DOI: https://doi.org/10.36660/ijcs.201810080 254 ORIGINAL ARTICLE International Journal of Cardiovascular Sciences. 2020; 33(3):254-262 Mailing Address: Silvia Maria Lucena Montenegro Instituto Aggeu Magalhães - Av. Professor Moraes Rego, s/n. Postal Code: 50.740-465, Recife, PE - Brazil. E-mail: silvia@cpqam.fiocruz.br Evaluation of Polymorphisms in IL8 and IL16 Genes in Patients with Acute Coronary Syndrome Lílian Caroliny Amorim Silva, 1 R omário Martins Araújo, 1 F ábia Carla Silva Soares, 1 Roberto Pereira Werkhauser, 1 S ergio Tavares Montenegro, 2 T etsuo Tashiro, 3 Viviane do Carmo Vasconcelos Carvalho, 1 S ilvia Maria Lucena Montenegro 1 Instituto Aggeu Magalhães, 1 Recife, PE - Brazil Real Hospital Português de Beneficência em Pernambuco (RHP), 2 Recife, PE - Brazil Universidade Federal de Pernambuco (UFPE), 3 Recife, PE - Brazil Manuscript received on February 08, 2018, revised manuscript on January 22, 2019, accepted on May 13, 2019. Abstract Background: Acute coronary syndrome (ACS) is a cardiovascular disease caused by obstruction of coronary arteries by atheromatous plaque. Susceptibility to this disease may be related to genetic variations, such as single nucleotide polymorphisms (SNPs). Objective: In this study, we evaluated the relationship between SNPs in IL8 (rs4073; -251 A/T) and IL16 (rs11556218; T/G) genes and SCA in a Brazilian population. Materials and Methods: A sample of 200 patients with ACS and 50 non-ACS patients hospitalized at the Real Hospital Português , Recife – PE, Brazil, and 220 blood donors (donors) was used. Genotyping was carried out by polymerase chain reaction, and DNA sequencing. Statistical analyzes were performed using the Williams G, Chi-square and Kruskal Wallis tests, using the BioEstat 5.0 program, and the data with a value of p < 0.05 were considered significant. Results: In the IL8 gene, the AT genotype was the most frequent (p > 0.05) in all three groups. In the IL16 gene, genotypic distributions were different between patients with ACS and the donor group (p = 0.002), with the most frequent G allele in the second group (p = 0.0052). The IL-16 cytokine was higher in donors than in patients with ACS (p = 0.04) and the G (TG + GG) allele had higher values of this cytokine (p = 0.01). Conclusions: The results demonstrate the important role of the rs11556218 SNP in IL16 gene in SCA, evidencing that the G allele may be associated with a decreased risk of the disease. (Int J Cardiovasc Sci. 2020; 33(3):254-262) Keywords: Cardiovascular Diseases; Acute Coronary Syndrome; Genotype; Dyslipidemia; Diabetes Mellitus; Obesity; Sedentarism. Introduction Acute coronary syndrome (ACS) is a cardiovascular disease (CD) characterized by occlusion of the coronary arteries and most often begins with the rupture of an atherosclerotic plaque in this artery, inducing the formation of a thrombus that occludes partially or totally the vessel. 1 Among the risk factors that influence its development, the presence of diabetes, dyslipidemia, smoking, obesity, sedentarism, hypertension and stress are highlighted. Age, gender and family history are also considered risk factors. 2 In addition, genetic factors may also contribute to a greater susceptibility to disease. 3 Some genetic polymorphisms have already been linked to the risk of developing CDs in specific populations. Interleukin (IL) -8 is produced by a variety