IJCS | Volume 32, Nº2, May/June 2019

250 Figure 1 - Letter: A, D, G) Histological section of the left ventricle stained with Hematoxylin and Eosin (HE), 40x increase in the Control Group (CG, n = 5), Low Concentration Group (LCG, n = 5) and High Concentration Group (HCG n = 5). B, E, H) Binarization process in the Control Group (CG, n = 5), Low Concentration Group (LCG, n = 5) and High Concentration Group (HCG n = 5). C, F, I) HE image after the binarization process in the Control Group (CG, n = 5), Low Concentration Group (LCG, n = 5) and High Concentration Group (HCG n = 5). The cell nuclei can be observed in black and the rest of the cell (cytoplasm, plasma membrane and other elements), in white. Figure 2 - Analysis of cardiomyocyte hypertrophy. CG: control group, LCG: low concentration group, HCG: high concentration group. (*p < 0.05 when comparing HCG versus CG). Micrometer (μm) CG LCG HCG Negrão et al. Effect of herbicide 2,4D inhalation on cardiac remodeling Int J Cardiovasc Sci. 2019;32(3)247-252 Original Article