IJCS | Volume 32, Nº2, May/June 2019

212 Figure 3 - Concentration-response curve for CLAE (1, 3, 10, 30, 100, 300 and 1000 µg/mL) in rat isolated thoracic aorta annulus with intact endothelium and pre-contracted endothelium with PHE (0.1 μM) before E + and after cyclooxygenase-1 (COX-1) inhibition. The dots represent the mean ± SD of the concentrations used with n = 6 and **(p < 0.01) versus E + . % RELAXATION AECL Present endothelium (EC 50 = 4.32 ± 0.07 µg/mL) After Indomethacin** (EC 50 = 124.60 ± 0.05 µg/mL) Figure 4 - Concentration-response curve for CLAE (1, 3, 10, 30, 100, 300 and 1000 µg/mL) in rat isolated thoracic aorta annulus with intact endothelium and pre-contracted endothelium with PHE (0.1 μM) before (E + control) and after incubation with atropine (1µM). The dots represent the mean ± SD of the concentrations used with n = 6 and **(p < 0.01) versus E + . % RELAXATION AECL Present endothelium (EC 50 = 4.32 ± 0.05 µg/mL) After atropine** (EC 50 = 437.10 ± 0.20 µg/mL) Brozzo et al. Curcuma longa abolishes contractions in the aortic artery Int J Cardiovasc Sci. 2019;32(3)207-216 Original Article