IJCS | Volume 31, Nº3, May/ June 2018

279 Camargo et al. Manual lymphatic drainage, natriuresis and lipolysis International Journal of Cardiovascular Sciences. 2018;31(3)274-281 Original Article Table 4 - Urinary glycerol excretion in young women, users (OCPu) or not (nOCPu) of oral contraceptives on manual lymphatic drainage (MLD) day or control (C) day at 0, 60 and 120 minutes 0 60 120 P** 60 vs 0 P # 120 vs 0 P & 120 vs 0 nOCPu C 0.02(0.01-0.03) 0.01(0.01-0.06) 0.02(0.01-0.07) 0.91 0.62 0.52 MLD 0.01(0.01-0.02) 0.03(0.01-0.08) 0.02(0.01-0.07) 0.10 0.19 0.80 OCPu C 0.05(0.02-0.15)* 0.10(0.04-0.31)* 0.17(0.06-0.56)* # 0.05 0.01 0.23 MLD 0.09(0.01-0.15)% 0.12(0.03-0.32)% 0.17(0.07-0.44) %# 0.10 0.01 0.38 Between- group analysis P* C 0.01 0.0001 0.0001 P MLD 0.0049 0.0012 0.0001 Within- group analysis nOCPu 0.59 0.14 0.72 OCPu 0.94 0.86 0.91 Normal distribution was tested by the Shapiro-Wilk test. Data without normal distribution were expressed as median and interquartile range (25%-75%), whereas those with normal distribution as mean ± standard error. For normally distributed data, the Mann-Whitney test was used for unpaired data (different “n”) and the Wilcoxon test for paired data (same “n”). The unpaired t-test was used for data that failed to pass the normality test. Significance level was set at 5%. Table 5 - Urinary atrial natriuretic peptide excretion in young women, users (OCPu) or not (nOCPu) of oral contraceptives on manual lymphatic drainage (MLD) day or control (C) day at 0, 60 and 120 minutes 0 60 P** 60 vs 0 nOCPu C 0.79(0.33-2.71) 1.10(0.54-3.71) 0.45 MLD 1.91(0.57-3.94) 1.72(0.88-4.79) 0.87 OCPu C 2.45(1.21-2.86) 4.05(1.90-6.63)* 0.05 MLD 2.38(1.63-4.92) 7.67±5.48%** 0.03 Between-group analysis P* C 0.06 0.0047 P% MLD 0.14 0.0054 Within-group analysis nOCPu 0.21 0.24 OCPu 0.32 0.13 Normal distribution was tested by the Shapiro-Wilk test. Data without normal distribution were expressed as median and interquartile range (25%-75%), whereas those with normal distribution as mean ± standard error. For normally distributed data, the Mann-Whitney test was used for unpaired data (different “n”) and the Wilcoxon test for paired data (same “n”). The unpaired t-test was used for data that failed to pass the normality test. Significance level was set at 5%. OCP inhibits renal sensitivity to vasopressin, requiring higher hormone production to obtain the same effects. This is corroborated by our results in OCPu on both days, suggesting that OCP induces greater sensitivity to central osmoreceptors. We believe that resting and temperature- controlled conditions in our study prevented water loss from the body surface, resulting in dilution in the extracellular compartment and inhibitory stimulus of vasopressin, which, in turn, promoted an increase in urinary excretion in the OCPu group. Increased venous return is one of the stimulus for ANP release by atrial cells. ANP exerts its biological