IJCS | Volume 31, Nº3, May/ June 2018

277 Camargo et al. Manual lymphatic drainage, natriuresis and lipolysis International Journal of Cardiovascular Sciences. 2018;31(3)274-281 Original Article Table 1 - Urinary composition in young women, users (OCPu) or not (nOCPu) of oral contraceptives on manual lymphatic drainage (MLD) day or control (C) day at baseline Urinary flow rate nOCPu OCPu Between- group analysis Within-group analysis C MLD C MLD P* C P # MLD P $ nOCPu P $ OCPu Urine (mL/min) 0.70(0.47-1.35) 1.20(0.70-1.93)$ 0.51(0.33-0.75)* 0.70(0.40-1.00) # 0.048 0.006 0.034 0.177 Sodium (mM/min) 140(110.50-184.20) 185.40±100 .00 113.30(78.05-149 .50)* 161.10±87.10 0.027 0.329 0.414 0.097 Glycerol (µmol/min) 0.01(0.007-0.035) 0.014(0.007-0.02) 0.04(0.01-0.15)* 0.09(0.01-0.14) # 0.014 0.004 0.595 0.945 ANP (pg/min) 0.79(0.33-2.71) 1.91(0.57-3.94) 2.45(1.21-2.86) 2.38(1.63-4.92) 0.064 0.146 0.214 0.324 Normal distribution was tested by the Shapiro-Wilk test. Data without normal distribution were expressed as median and interquartile range (25%-75%), whereas those with normal distribution as mean ± standard error. For normally distributed data, the Mann-Whitney test was used for unpaired data (different “n”) and the Wilcoxon test for paired data (same “n”). The unpaired t-test was used for data that failed to pass the normality test. Significance level was set at 5%. ANP: atrial natriuretic peptide. Urinary flow remained unchanged in nOCPu on both days. However, on control day, OCPs showed an increase in this parameter over time. On MLD day, although urinary flow increased from 0 to 60min, no difference was observed between 120 min and 60 minutes (Table 2). Urinary excretion of sodium increased in nOCPu group on control day only at 120 minutes; however, on MLD day, this increase occurred at 60 min, indicating an acute effect of the therapy on sodium in the body. At 0 on control day, sodiumurinary excretionwas lower inOCPu Table 2 - Urinary flow rate in young women, users (OCPu) or not (nOCPu) of oral contraceptives (OCPu) on manual lymphatic drainage (MLD) day or control (C) day at 0, 60 and 120 minutes 0 60 120 P** 60 vs 0 P # 120 vs 0 P & 120 vs 60 nOCPu C 0.70(0.47-1.35) 1.00(0.70-1.31) 1.00(0.69-1.47) 0.06 0.32 0.38 MLD 0.70(0.49-1.90) 1.20(0.70-1.93) 1.20(0.59-1.40) 0.08 0.86 0.15 OCPu C 0.51(0.33-0.75)* 0.83(0.58-1.47)** 1.18(0.84-1.80) #& 0.0001 0.0001 0.01 MLD 0.70(0.40-1.00) 1.30(0.69-2.25)** 1.40(0.96-1.80) # 0.0001 0.0009 0.56 Between- group analysis P* C 0.04 0.51 0.19 P MLD 0.27 0.75 0.07 Within- group analysis nOCPu 0.18 0.32 0.91 OCPu 0.29 0.13 0.94 Normal distribution was tested by the Shapiro-Wilk test. Data without normal distribution were expressed as median and interquartile range (25%-75%), whereas those with normal distribution as mean ± standard error. For normally distributed data, the Mann-Whitney test was used for unpaired data (different “n”) and the Wilcoxon test for paired data (same “n”). The unpaired t-test was used for data that failed to pass the normality test. Significance level was set at 5%.