IJCS | Volume 31, Nº3, May/ June 2018

DOI: 10.5935/2359-4802.20180023 274 ORIGINAL ARTICLE International Journal of Cardiovascular Sciences. 2018;31(3)274-281 Mailing Address: Maria Silvia Mariani Pires-de-Campos Rua do Rosário, 1260. Postal Code: 13400-000, Piracicaba, São Paulo, SP - Brazil. E-mail: mscampos@unimep.br Acute Effect of Manual Lymphatic Drainage on Natriuresis and Lipolysis in YoungWomen Érica A. M. Camargo 1 ; Filipy Borghi 1 ; Aglécio Luiz de Souza 2 ; Denise Maria Marcorin 3 ; Luana de Lima Rodrigues 3 ; Danilo Roberto Xavier de Oliveira Crege 4 ; LarissaYuri Ishizu 1 ; Priscila Cristina da Silva 1 ; DoraMaria Grassi-Kassisse 1 ; Maria Silvia Mariani Pires-de-Campos 3 Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), 1 São Paulo, SP - Brazil Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), 2 São Paulo, SP - Brazil Faculdade de Ciências da Saúde (FACIS), Universidade Metodista de Piracicaba (UNIMEP), 3 São Paulo, SP - Brazil Centro Universitário Padre Anchieta, 4 Jundiaí, SP- Brasil Manuscript received May 18, 2017; revised manuscript October 16, 2017; accepted November 13, 2017. Abstract Background: The importance of scientific validation of supporting techniques to various treatments is unquestionable. In this context, the influence of manual lymphatic drainage (MLD) on natriuresis and lipolysis and its interaction with oral contraceptives still need to be investigated. Objectives: To evaluate the acute effect of MLD on natriuresis and lipolysis in young women using or not oral contraceptives. Methods: Twenty-nine non-users of oral contraceptives and 29 oral contraceptive users, self-reported healthy, sedentary, normal weight women were enrolled. Analyses were conducted on two different days – control (C), without therapeutic intervention and MLD day. Four urine samples were collected at 60-minute intervals. MLD was performed in lower limbs and abdomen for 45 min following the Leduc method. Urinary flow rate and urinary sodium, glycerol and atrial natriuretic peptide excretion were analyzed. Data normality was tested by the Shapiro- Wilk test. Data without normal distribution were expressed as median and interquartile range (25%-75%), while normally distributed data were expressed as mean ± standard error. Mann-Whitney test was used for unpaired data and Wilcoxon test for paired data. Data with normal distribution were evaluated by the unpaired t-Student test. Statistical significance was set at 5%. Results: One MLD session had an acute effect on both groups, increasing natriuresis in non-users of oral contraceptives and glycerol and atrial natriuretic peptide excretion in oral contraceptive users. Conclusion: Oral contraceptives influence the effect of MLD on natriuresis. (International Journal of Cardiovascular Sciences. 2018;31(3)274-281) Keywords: Musculoskeletal Manipulations; Natriuresis; Lipolysis; Lymphatic System. Introduction Manual lymphatic drainage (MLD) was created by the Danish physicianDr. Emil Vodder in 1936 as a supporting therapy, later established as the gold standard for the treatment of lymphedema. 1 However, based on beauty standards currently imposed by society, many women undergo some types of treatments that have no scientific basis for their well-being. In this context, MLD has become a popular procedure among healthy individuals to reduce body size, and performed by individuals without knowledge about lymphatic system physiology or pathophysiology. Lymphatic system has a crucial role in maintaining fluid balance in the body, macromolecular homeostasis, lipid absorption and immune function. However, the effect of MLD on other systems has been poorly investigated. 1