ABC | Volume 115, Nº2, August 2020

Original Article Basilio et al. Effects of intermittent fasting on the heart Arq Bras Cardiol. 2020; 115(2):184-193 Figure 3 – (A) Cross-sectional area of the cardiomyocytes (CSA); † p<0.05 versus IF; ‡ p<0.05 vs. ET. Two-way ANOVA and Student-Newman-Keuls test. (B) Frequency distribution of cardiomyocytes according to CSA class. Classes: I (52.7┤190.1 µm 2 ), II (190.1┤327.6 µm 2 ), III (327.6┤465.0 µm 2 ), IV (465.0┤602.4 µm 2 ) and V (602.4┤739.9 µm 2 ); * p<0.05 vs. Control; † p<0.05 vs. IF; ‡ p<0.05 vs. ET. Goodman test for contrasts within and between multinomial populations. Control, sedentary rats receiving the control diet ad libitum; IF: sedentary rats undergoing intermittent fasting; ET: rats exercising and receiving the control diet ad libitum; ETI: rats exercising while intermittent fasting. Table 3 – Protein levels of MAPK isoforms in myocardial tissue Protein Group Control n=6 IF n=6 ET n=6 ETI n=6 p-ERK/ERK 1.00 ± 0.52 1.42 ± 1.59 1.08 ± 0.48 1.23 ± 0.78 p-ERK/GAPDH 1.00 ± 0.47 1.18 ± 1.19 0.87 ± 0.41 0.86 ± 0.32 ERK/GAPDH 1.00 ± 0.10 0.99 ± 0.23 0.91 ± 0.16 0.91 ± 0.22 p-JNK/JNK 1.00 ± 0.39 1.00 ± 0.35 1.09 ± 0.61 1.03 ± 0.40 p-JNK/GAPDH 1.00 ± 0.13 1.11 ± 0.26 1.10 ± 0.35 1.15 ± 0.23 JNK/GAPDH 1.00 ± 0.46 1.04 ± 0.42 0.94 ± 0.31 0.96 ± 0.32 Values expressed as mean ± standard deviation. ERK: extracellular signal-regulated kinase; JNK: c-Jun N-terminal kinase. Two-Way ANOVA (p>0.05). 190