ABC | Volume 114, Nº2, February 2020

Original Article Stefani et al. DNA damage and heart failure Arq Bras Cardiol. 2020; 114(2):234-242 1. McMurray JJV, Adamopoulos S, Anker SD, Auricchio A, BöhmM, Dickstein K, et al. ESCGuidelines for the diagnosis and treatment of acute and chronic heart failure 2012: The Task Force for the Diagnosis and Treatment of Acute and Chronic Heart Failure 2012 of the European Society of Cardiology. Developed in collaborationwith theHeart. Eur Heart J. 2012;14(8):803–69. 2. Yamaguchi T, Izumi Y, Nakamura Y, Yamazaki T, Shiota M, Sano S, et al. Repeated remote ischemic conditioning attenuates left ventricular remodeling via exosome-mediated intercellular communication on chronic heart failure after myocardial infarction. Int J Cardiol. 2015 Jan 15;178:239–46. 3. ZizolaC,SchulzePC.Metabolicandstructural impairmentofskeletalmuscle in heart failure. Heart Fail Rev. 2013;18(5):623–30. 4. Tsutsui H, Kinugawa S, Matsushima S. Oxidative stress and heart failure. 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References might lead to a more robust scenario of DNA damage and its lack of repair of DNA lesions. Our design aims to evaluate the longitudinal damage that the experimental model of CHF might lead to and its differences regarding tissues; thus, the ability of DNA to repair its lesions could not be carried out. Conclusion Our results show DNA damage using SCGE in the CHF experimental model byMI. The left ventricle dysfunction clearly affects the cardiac tissue, lungs, diaphragm, gastrocnemius and soleus and was associated with the extent of DNA damage, affecting the soleus muscle more than the left ventricle and the gastrocnemius. The comet assay was proven to be a reliable tool for quantifying DNA damage in different tissues of animals with CHF and the soleus muscle was shown to be more affected by the heart failure than the left ventricle and the gastrocnemius. Author contributions Conception and design of the research, Acquisition of data, Analysis and interpretation of the data, Writing of the manuscript and Critical revision of the manuscript for intellectual content: Stefani GP, Nunes RB, Rossato DD, Hentschke VS, Di Domenico M, Dal Lago P, Rhoden CR; Statistical analysis: Stefani GP, Nunes RB, Rossato DD, Hentschke VS, Dal Lago P. Potential Conflict of Interest No potential conflict of interest relevant to this article was reported. Sources of Funding This study was funded by Capes, CNPq, UFCSPA. Study Association This article is part of the thesis of master submitted by Giuseppe Potrick Stefani, from Universidade Federal de Ciências da Saúde de Porto Alegre. Ethics approval and consent to participate This study was approved by the Ethics Committee of the CEUA/UFCSPA under the protocol number 114/13. All the procedures in this study were in accordance with the 1975 Helsinki Declaration, updated in 2013. 240