ABC | Volume 114, Nº2, February 2020

Original Article Fleury et al. Epinephrine and felypressin effects Arq Bras Cardiol. 2020; 114(2):295-303 Table 2 – Response Duration Number of cartridges Normotensive Hypertensive Atenolol-treated rats Nonsmoker Smoker Nonsmoker Smoker Nonsmoker Smoker Epinephrine Felypressin Epinephrine Felypressin Epinephrine Felypressin Epinephrine Felypressin Epinephrine Felypressin Epinephrine Felypressin 1 453.5 (412-517) 905.2 (748,6-945)* 276.5 (214-298) 908 (880-948)* 297 (278-333) 911.5 (880-949)* 325.5 (277-492) 818.5 (610-876)* 291.5 (260-352) 811 (754-914)* 426 (382-470) 692.5 (552-738)* 2 427 (399-471) 1,042 (928.2-1118.9)* 268.5 (256-302) 888.5 (796-947)* 421 (376-436) 979.5 (934-1012)* 344 (329-497) 875.5 (771-972)* 398 (345-444) 545 (496-708)* 498 (413-616) 960 (920-976)* 4 445 (378-466) 1,117 (1,050-1,184.4)* 287 (294-323) 1,004.5 (876-1,086)* 422.5 (351-464) 1,021 (920-1,080)* 475 (357-513) 954.5 (882-1,042)* 415.5 (380-453) 712 (552-858)* 421 (405-464) 938 (783-1,111)* 8 455 (423-506) 1,077.1 (983-1,193)* 353 (332-365) 1,066.5 (1,000-1,170)* 455 (425-538) 1,032.5 (1,011-1,229)* 472.5 (400-507) 1,087 (998-1,154)* 454 (393-489) 946 (800-1,103)* 511.5 (479-586) 1,063.5 (987-1,240)* Response duration (in seconds) after intravascular administration of epinephrine or felypressin (dose contained in the corresponding number of local anesthetic cartridges) in smoker and non-smoker normotensive, 1K1C hypertensive and 1K1C atenolol-treated groups (n = 6 for all groups). Median (25 th Percentile-75 th Percentile). * p < 0.05 compared with epinephrine. population 13 , our results suggest that the hypertensive response to epinephrine in hypertensive smoker and non-smoker rats without atenolol treatment, remains unchanged. On the other hand, the hypertensive, smoker and atenolol-treated rats showed significantly higher blood pressure measurements when compared with non-treated animals. It is still not clear if cigaretes smoking increases blood pressure values, as some studies indicted that it can potentiate family history and increase systolic, diastolic and mean arterial pressure value. 6 . In our study, the smokers groups did not show increased basal blood pressure values when compared with non-smokers (Table 1). Smoke exposure was carried out for 4 weeks, as expected, and this time was sufficient to alter vasoconstrictor response. Rats were submitted to an epinephrine or felypressin intravascular injection under ketamine/xylazine anesthesia; it was shown in a previous study Figure 5 – Comparison of maximal hypertensive response curves after intravenous injection in bolus of epinephrine and felypressin in control non-smoker normotensive, smoker normotensive rats, non-smoker 1K1C hypertensive, smoker 1K1C hypertensive, non-smoker 1K1C atenolol-treated and smoker 1K1C atenolol-treated rats. n = 6 for all groups. Median (25 th Percentile-75 th Percentile). *p < 0.05 in the comparison between drugs. 300