ABC | Volume 114, Nº1, January 2019

Original Article Brianezi et al. Effects of physical training on the myocardium Arq Bras Cardiol. 2020; 114(1):100-105 Table 1 – Antibodies used for protein detection Antibody Dilution Labeling Function Manufacturer MMP2 1:250 Cytoplasm Collagen remodelling sc-10736, Santa Cruz® MMP9 1:300 Cytoplasm Collagen remodelling sc-6840, Santa Cruz® COX 2 1:100 Cytoplasm Inflammation sc-1745 P, Santa Cruz® 8-OHdG 1:100 Cytoplasm Oxidative stress sc-66036, Santa Cruz® Statistical analysis Absolute and relative values were used to describe the qualitative variables. For the quantitative variables with normal distribution (Shapiro-Wilk > 0.05), mean, standard deviation, minimum and maximumwere used, and for the variables with non-normal distribution (Shapiro-Wilk < 0.05), median and percentiles were used. To study the diferences between the variables with the groups, the Kruskal-Wallis and ANOVA tests were used, complemented by the Dunn test or Bonferroni. For all analyzes, a confidence level of 95% was used. The program utilized for these analyzes was Stata version 11.0. (StataCorp. 2009. Stata Statistical Software: Release 11 . College Station, TX: StataCorp LP.) Results Our data show a significant reduction in the volume density of type I collagen fibers in the ovariectomized dyslipidemic group (LDL-OS) (-51%). The LDL-OT group showed a decrease of 100% when compared to the COS group and a significant difference in relation to the COT and LDL-S groups. Table 2 shows the association of COX-2 and MMP9 expression variables and the density of type I collagen fibers in relation to the evaluated animal group. Regarding metalloproteinase type 2 expression, in the control group (CS) there was a significant increase of 24% in the ovariectomized group (COS), when physical training (COT) was performed there was an increase of 72%, and with dyslipidemia (LDL-S), the increase was 92%. In the dyslipidemic group with ovariectomy (LDL-OS), the values decrease 27% in relation to the LDL-S group and 18% in relation to the COT group, but still above the values of the control groups by 42% in relation to the CS group and 14% in relation to the COS group. When the training was performed in the ovariectomized dyslipidemic group LDL-OT, the results obtained present a 15% increase in relation to the sedentary ovariectomized LDL-OS group and a 15% decrease in relation to the LDL-S group. When compared to the control group, there was an increase of 64% in relation to the CS group, 32% in relation to the COS group and a decrease of 5% in relation to the COT group. When analyzing the oxidative stress data, evaluating 8OHdG expression, there was a decrease in the indexes for the control group (CS), in which there was a decrease of 33% in the group with ovariectomy (COT) and a 39% decrease in the trained group. When dyslipidemia was present (LDL-S), there was a 51% decrease in relation to the CS group, and in the dyslipidemic group with ovariectomy (LDL-OS) there was a decrease of 85% in relation to the CS group, 78% Figure 1 – Immunoexpression of proteins. CS: sedentary non-ovariectomized control; COS: sedentary ovariectomized control; COT: trained ovariectomized control; LDLr-S non-sedentary ovariectomized knockout; LLr-OS: sedentary ovariectomized LDL knockout; LDLr-OT: trained ovariectomized LDL knockout. 102