ABC | Volume 113, Nº1, July 2019

Original Article Wollmann et al Characterization of decellularized pericardium Arq Bras Cardiol. 2019; 113(1):11-17 Figure 3 – Graphical representation of the mechanical parameters obtained for fresh and decellularized human pericardium. (A) Tensile strength [MPa]; (B) elongation [%]; (C) elastic Modulus [MPa] and (D) the curves indicate the mean stress-strain behavior of fresh and decellularized human pericardium A B C D Fresh Decellularized 8 40 30 20 10 0 6 4 2 0 50 4 3 2 1 0 40 30 20 10 0 Fresh Tensile Strength [MPa] Elongation [%] Elastic modulus [MPa] Stress [MPa] Decell Fresh Strain [mm/mm] Decell Fresh Decell 0.00 0.05 0.10 0.15 0.20 0.25 Figure 2 – Agar overlay cytotoxicity assay. Testing image after 24 hours in contact with agar-overlay. Decellularized human pericardium (A), negative control (B) and positive control (C). Table 1 – Collagen, glycosaminoglycan and DNA content of fresh and decellularized human pericardium Collagen µg/mg Glycosaminoglycan µg/mg DNA ng/mg Fresh pericardium 126.9 ± 45.8 0.64 ± 0.50 1591 ± 726.0 Decellularized pericardium 138.3 ± 58.6 0.02 ± 0.06 511.23 ± 120.4 P value 0.716 0.014 < 0.001 15